Genetic DNA polymorphism induced by samarium (Sm) in the macrophyta Ulva lactuca alga as revealed by TU-DAMD marker

      Basel Saleh

      Volume 30, Issue 3;
      Pages: 274-281; 2024
      ISSN: 2069-0053 (print), Agroprint;
      ISSN (online): 2068-9551

Abstract

DNA change profile in the green Ulva lactuca alga after 2 days of exposure to different Samarium (Sm) (0.25, 0.5, 0.75 and 1 mg/L) concentrations, has been evaluated using touch-up directed amplification of minisatellite DNA (TU-DAMD) marker. Genomic template stability (GTS%) and band-sharing index (BSI) values as qualitative measure of DNA changes followed Sm treatment were calculated under the above applied Sm concentrations. Data showed that GTS% and BSI values followed similar tendency; in this regards, GTS% value increased from 37.891 to 50.865%, whereas, BSI value increased from 0.505 to 0.581 as Sm applied concentration increased from 0.25 to 1 mg/L Sm. The present study could be considered as the first report describes genomic instability induced by Sm in U. lactuca alga. Indeed, TU-DAMD marker could serve as a potent tool for monitoring genomic instability induced by Sm in U. lactuca alga in ecosystems. This data revealed new insight concerning genomic instability induced by Sm on marine algae.