Functional cooperation between jasmonic acid and PR3 signaling pathways during barley-Cochliobolus sativus interaction


M. Jawhar*, E. Al-Shehadah, A, Shoaib,  N. Moursel, Al-Daoude and MIE. Arabi

Volume 28, Issue 3;
Pages: 218-222; 2022
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551


Spot blotch (SB) caused by the fungus Cochliobolus sativus is a destructive disease of barley worldwide. Functional cooperation between Jasmonic acid (JA) and pathogenesis-related 3 (PR3) gene signaling pathways in barley with SB have been poorly documented. Keeping in view this objective, SB–resistant ‘Banteng’ and susceptible ‘WI2291’ genotypes were evaluated using qRT-PCR across four-time points after pathogen challenge. Data showed significant variance in the expression patterns of JA and PR3 between inoculated and non-inoculated plants in both genotypes, and their expressions were higher and faster in the resistant cultivar ‘Banteng’ as compared with the susceptible one ‘WI2291’ at each infection time point. However, qRT-PCR showed that 24 h constitute a significant starting time-point for PR3 and JA in demonstrating the differential response of both genotypes towards C. sativus. The maximum expressions were recorded for PR3 (5.43 and 2.81-fold) at 48 hours post inoculation (hpi) and for JA (152 and 142 ng/g) in the resistant and susceptible genotypes respectively, at 72 hpi. Taken together, our results confirm the importance of PR3 and JA -dependent signaling during barley- C. sativus interactions.

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