Determination of lactose in milk and dairy products by HPLC-RID method

Authors:

Sandra M. Jakšić *, Nenad S. Popov, Željko A. Mihaljev, Milica M. Živkov Baloš


Volume 28, Issue 1;
Pages: 5-10; 2022
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551

Abstract:

Fast, simple high-performance liquid chromatography (HPLC) method with refractive index detection (RID) was optimized and validated for the quantification of lactose in milk and dairy products. The method showed good linearity with determination coefficient 0.9975. The limit of detection was 0.04 mg/mL, and the limit of quantification 0.12 mg/mL. The relative standard deviations for repeatability were 4.00 and for reproducibility 4.98 in reference material, while in different tested matrix the relative standard deviations for repeatability were in range 2.58‒6.51. Average efficiency value was 97%. The applicability of the method was confirmed by its application in different samples. The presence of this sugar was not detected (lower than 0.1%) in the samples of milk and yogurt labelled as lactose-free, as well as in parmesan, cheddar, gouda and trappist cheese samples. In various dairy products the measured lactose content had values from 0.493±0.026% in home-made rolled cheese to 4.42±0.180% in cooking sour cream.

Location
300645-Timisoara, Calea Aradului 119, Romania
Phone
Tel: +40-256-277423
Fax: +40-256-277326
E-mail
nico_hadaruga@yahoo.com
info@journal-of-agroalimentary.ro
Copyright © 2022 Journal of Agroalimentary Processes and Technologies