Diversity of 16S rRNA gene and its GC contents in Bacillus species


M. Harba, M. Jawhar, M.I.E. Arabi

Volume 27, Issue 4S;
Pages: 565-569; 2021
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551


The 16S ribosomal RNA (rRNA) gene is widely used for estimating the composition of bacterial communities. In this work, the phylogenetic diversity of 40 Bacillus sp. strains  (3 B. atrophaeus, 20 B. subtilis, 2 Paenibacillus polymyxa, 10 B. amyloliquefaciens, 1 B. simplex and 4 B. tequilensis) were investigated based on 16S rRNA gene and its guanine (G) and cytosine (C) contents. 16S rRNA partial sequencing assigned all the strains to the Bacillus genus, with close genetic relatedness to the B. subtilis and B. amyloliquefaciens groups, and to the B. tequilensis. The phylogenetic tree did not result in any clusters/clades specific to origin or morphological criteria. In addition, the percentage of GC contents for 16S rRNA gene sequences ranged between 54.1% - 56.9%, and no clear correlation were observed between the GC content and distribution of species strains within clusters. This work demonstrated many of the same general features seen in the Bacillus species 16S rRNAs sequences.

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