Bacterial growth and competition status of Escherichia coli and Staphylococcus aureus in different semen media at two temperature degrees


M. Abboudi, S. Saoud, M. Alomar

Volume 27, Issue 3;
Pages: 326-335; 2021
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551


Bacterial growth and competition of (Escherichia coli and Staphylococcus aureus (were assessed in different semen preservation media at two temperature degrees. Five media were prepared (Tris solution, Tris solutions supplemented with: glucose, fructose and sucrose, and Tris solution with egg-yolk. Radiation by gamma ray (1KGy) was used to sterilize these media. The species were incubated at 103 CFU/mL for 3 hours at 4 and 37°C. The storage of the two species in egg yolk medium at 37°C had a clear significant effect on increasing bacterial loads compared to 4°C, whereas it reached approximately 150 and 300% for S.aureus and E.coli respectively. When S.aureus was co-incubated with E.coli in different semen media, a strong ability of S.aureus to dominate E.coli growth was noticed at 4°C. Surprisingly it was completely able to stop E.coli growth at 37°C. It was obvious, that each bacterium is unique and has specific requirements. The competition between E.coli and S.aureus in semen media could be related to the initial load of contamination and the time of incubation but most importantly to the media composition and temperature degree.

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