Heterogeneity within the 16S rRNA gene of Bacillus subtilis isolates differing with antagonistic activity against Cochliobolus sativus pathogen

M. Harba, M. Jawhar, M.I.E. Arabi

Volume 27, Issue 2;
Pages: 88-93; 2021
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Bacillus subtilis, a gram-positive bacterium, has attracted considerable interest in both academic and industrial research. However, despite its substantial importance, there is a lack of appropriate and adequate information regarding the genetic diversity of its isolates. In this work, the nucleotide sequences of the 16S ribosomal RNA (16S rRNA) of 19 B. subtilis isolates differing in antagonistic activity were examined. The results indicated that 16S rRNA gene had sequence variations at 14 positions in the 514 bp including insertions, deletions and SNPs. 16S rRNA data clearly divided isolates of B. subtilis into two major clades. Clade 1 contained major sub-clusters consisting of 14 isolates, whereas the remaining 5 isolates were placed in clade 2. On the other hand, B. subtilis had significant antagonistic activities against the barley fungal pathogen Cochliobolus sativus, and 16S rRNA data did not result in any clusters/clades specific to antagonism. The molecular parameter used in this study provides crucial information about the genetic heterogeneity among16S rRNA gene of B. subtilis isolates which can have a profound effect on the classification of taxa.
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