Treating the biological waste used in bacterial culture is so important to save the environment and this can be useful for laboratories in countries with limited resources, which means financial benefit. The investigation of the bacterial growth on the used media recycled was studied in this work. The general media (plate count agar) can be reused twice after the recycling to cultivate and identify E. coli, S. aureus, Salmonella sp, and B. subtilis by the spread method on the plate count agar medium. The size of bacterial colonies decreased in the second step of the recycling from -30 to -60% in comparison with the control. The processes to recycle the used media help transform it to new powder that could be stored for a long time (up to six months). Three methods of additional sterilization were tested on the used media: (1) the sterilization by gamma irradiation at 5 kGy, (2) the microwave for 8 min, and (3) the hot drying in oven at 80 °C. Using the microwave radiation during the processes of recycling allowed having new media, with color and clarity similar of the fresh media and available in laboratories.
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