Anethum graveolens L. (dill) is a popular aromatic herb frequently used as a spice but also for its healing properties which are associated with antioxidant, anti-inflammatory, antibacterial and antiviral actions exerted by the synergistic action of classes of bioactive compounds present in the composition. The main purpose of this study was to evaluate the antibacterial action and cytotoxic potential of a crude aqueous extract of dill seeds. The antibacterial potential determined by the disc diffusion method was observed against E coli. Regarding the cytotoxic activity, evaluated by the MTT method, a reduction of the number of living cells was observed when using the highest concentrations on the SCC-4 tumor line, while at the same healthy line concentrations, PGK was not affected. In-depth studies related to the pharmacological activity of the extract must be carried out in order to be able to decipher the mechanisms of antibacterial or antitumor action.