The experiment was conducted on cherry kernels treated with gamma irradiation (with the dose of 0, 3 and 6 kGy) and stored up to 12 months at room temperature to estimate the effect on proximate, quality, and microbial changes of cherry seed kernel (ChSK). The results showed the abundance (%) of approximate parameters evaluated to be in fresh matter, crud fat (35.84%) > total sugar (30.34%) > crud protein (23.47%) > moisture (4.51%) %) > ash (3.51%) > total reducing sugar (2.11%) order. in general, there are no appreciable differences in proximate composition of ChSK samples irradiated with 0, 3, and 6 kGy and stored for 12 months. Gamma irradiation had no effect on acidity, pH and volatile basic nitrogen values of CHSK. The initial total viable count (TVC), total mould and yeast count (TMYC), and total coliform count (TCC) were 4.20 ±0.07, 4.52 ±0.20, and 2.56 ± 0.17 log CFU g-1. TVC, TCC, and TYMC was significantly (P<0.05) reduced with irradiation (from 0 to 6 kGy). Considering all traits it can be concluded that gamma irradiation treatments significantly reduced microbial population and maintain chemical composition and quality of ChSK.