The oil content and fatty acid compositions of some edible macrofungi (Agaricus campestris, Boletopsis leucomelaena, Chroogomphus helveticus, Chroogomphus rutilus, Coprinus micaceus, Lactarius deliciosus, Lactarius semisanguifluus, Lactarius vellereus, Lepista nuda, Morchella conica, Morchella esculenta, Rhizopogon roseolus, Suillus luteus, Terfezia boudieri and Terfezia sp., ) growing in Turkey were determined. The oil content and free fatty acidity values of the fresh macrofungi samples were found between 0.47-2.71 g and 0.015-0.087 g, respectively. The most abundant fatty acids were linoleic, palmitic and oleic acids in 12 taxa, two taxa and one taxon macrofungi oils, respectively. Total unsaturated fatty acid ratios of different species were found between 44.82 % and 79.76 %. The highest unsaturated fatty acids were found in the composition of Terfezia sp.