Induced breeding of H. molitrix and histological observations on the development stage of oocyte

Adina Popescu, Mirela Crețu, Aurelia Nica, Daniela Cristina Ibănescu

Volume 24, Issue 4;
Pages: 325-329; 2018
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
The aim of the present study was to induced breeding of H. molitrix and to observe gonads maturation after the injections with the synthetic hormone Nerestin. Males and females of H. molitrix were provided from the Cârja farm 1-from Vaslui county. All the breedings were injected with Nerestin 1, the females received two doses of 20%, respectively 80% of the total dose, within 24 hours, and the males received only the two doses representing 1/3 and 2/3 of the dose total administered to females. For histological examination, oocyte samples were collected from adult H. molitrix females. The sampling periods overlapped with the maturation period after the vitellogenesis (April-May 2016) and ovulation, which took place in June 2016. The ovulation process is determined by internal factors and assured by the normal development of the body; in the case of induced breeding is triggered by the administered gonadotrophic stimulants. Our results showed that in order to obtain good quality eggs a great importance must be given to the time of administration of gonadotrophin stimulants, and the quantity and quality of the introduced substance. The biological material taken for the histological examination was processed by classical methods. Fixation was done in Bouin and Formalin, and after inclusion in paraffin, the pieces were cut at 7 μm by microtome Slee. During maturation period, the oocytes gradually pass into stage VIIB, the nucleus migrates to the animal pole in the cytoplasmic area at the micropyle. During the ovulation period, the histological sections show the presence of oocytes matures with finished vitellogenesis, prior to expulsion from ovary. Histological, it was noted the evidence of the oocytes in stages VIIB and VIIC. Oocytes remain at this stage for 20-25 days. It is the optimal period of hormonal induction until the beating conditions are formed.
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