Protein and amino acid profiling by the Cyanobacterium Spirulina (Arthrospira) platensis Strains in different Inorganic Formulations

Gajendra Kumar Dautania, Gajendra Pal Singh

Volume 24, Issue 3;
Pages: 143-151; 2018
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Spirulina platensis is a microscopic, filamentous and alkalophilic fresh water microalga isolated from Jalmahal Lake, Jaipur (Rajasthan), India. The biochemical constituent of cyanobacteria depends on the nature of strains, nutrient composition, physiological state of the culture and the environment. To find out the best inorganic composition cultures were grown in five different defined inorganic medium and kept at the temperature of 25 ± 2°C, illuminated with white fluorescent lamps at a light intensity of 2,500 lux with photoperiod of 12 hours light/dark cycle. Maximum protein content was observed in Zarrouk’s media. In SDS-PAGE profile total 12 polypeptides were observed in which three protein bands having molecular weight of 52.1 kDa, 105.2 kDa, and 110.0 kDa which has not been reported in cultures grown in Kratz and Myer medium. Total 17 amino acids were observed in chromatogram of HPLC and paper chromatography in cultures grown in Zarrouk’s medium. Cysteine, histidine, methionine and serine were absent in CHU 10 medium and Kratz and Myer medium. Asparagine, glutamine and tryptophan were never observed in all inorganic formulations.
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