Milk whey proteins and onion extract powder interactions - antimicrobial and anticancer activities

Authors:
Mostafa Ali, Essam Elsebaie

Volume 24, Issue 3;
Pages: 152-160; 2018
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Abstract:
The effect of modification of milk whey protein (WP) with onion extract and quercetin on their antimicrobial and anticancer activities was studied .WP was modified with onion extract powder rich with quercetin (OE) and quercetin dihydrate (Q) with concentration of (60 mg/g protein) at alkaline conditions (pH 9) and room temperature in the presence of air for 24 h. The findings showed that the quercetin of OE and Q was covalently attached to WP as illustrated by RP-HPLC and the decrease in tryptophan content. In addition, the derivatives produced between WP, OE and Q were characterized in terms of their antimicrobial and anticancer properties. The results displayed that, WP modified with OE or Q showed a significant antimicrobial activity especially against gram positive bacteria (Bacillus cereus ) compared to unmodified WP (control ). WP modified with Q was more effective than other investigated samples. Moreover, WP modified with OE and Q showed a significant anticancer activity especially against H1299 (carcenogenic cells of human lung). The activity of WP modified with Q against MCF7-1(carcenogenic cells of human breast) and H1299 (carcenogenic cells of human lung) was higher than those of WP modified with OE where their IC50 values were13.6µg/ml and 18.7µg/ml, respectively. Therefore, this study suggests that these proteins may have potential alternative antimicrobial and anticancer properties as functional ingredients, so they can be used in preparing functional and nutraceutical foods.
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