Cr, Mn, Fe, Co, Ni , Cu, Cd, Pb, As and Zn contents of cumin seeds were found as 0.298, mg/kg, 16.208, 95.279, 0.101, 1.545, 7.458, 0.037, 0.051, 0.109 and 29.183, respectively. As physico-chemical properties of cumin essential oil, oil yield, refractive index, viscosity, and ransimat value were measured as 1.6%, 1.506, 3.37, and 2.25 (110 oC-h; µs/cm-h). Cumin oil contained 65.12% oleic, 29.25% linoleic and 3.51% palmitic acids as major fatty acid. Twenty-eight constituents were identified in the oil of C.cyminum seed oil, representing 99.998% of the total oil. Cuminal (25.59%), (r)-(+)-1-phenyl-1-propanol (22.112%), 1-isopropylidene-3-n-butyl-2-cyclobutene (17.006%), gamma-terpinene (11.029 %), trans-beta-ocimene (7.952%) and β-pinene (5.81%) were established as major constituents of cumin seed essential oil. The highest antifungal activity was observed on mycelia growing of Aspergillus parasiticus NRRL 3357. Cumin oil showed weak effect on the mycelia growt of Zygosacc haromycesrouxii. S.aureus showed resistance against cumin oil when compared with results of S.enteritis, E.coli, L.monocytogenes and E.coli O157:H7. Cumin oil exhibited antimicrobial activity against the investigated test organisms.
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