Liver is an excellent source of all the biological molecules like essential, nonessential amino acids and long change omega 6 and omega 3 fatty acids. The objective of present study was to comparison the nutrition value of liver samples donkey, dog, camel, beef and goat by using standard methods. All the samples shown good content of amino acids, fatty acids, total protein content and moisture, also data illustrated that the differences between all samples understudy were significant (p<0.05). Glutamic acid is present in liver in the highest amounts (9.16%) of non-essential amino acids, followed by aspartic acid, cysteine was present in the lowest amount in all types of livers. Leucine was present in the highest percent among of essential amino acids followed by lysine. Camel liver have the highest value of myristic acid (3.11%) and palmitic acid (33.29%), beef liver have the highest value of margaric acids (3.26%) and stearic acid (42.93%). gamma-Linolenic acid was absent only in dog s and donkey s livers. The high level of protein (67,47% dw) and low level of crude fat (6.49% dw) were determined in donkey liver, while the highest level of fat (11.69%) were determined in goat liver (P < 0.05). According to the types of liver, protein content differs but not as much as fat content which ranged from 11.69 to 6.49% (P < 0.05). The moisture content was almost equal (70-71%) in all kind of liver samples except goat which had 67.70 % moisture