Fatty acid composition, total phenol and antioxidant activities of pumpkin seeds were determined. While genotype 109 has the highest seed yield (144.2 kg/da), genotype 106 had the lowest seed yield (42.9 kg/da). In addition, genotype 109 had the highest yield of seed (43.74 g/da) and yield of seed per plant (57.70 g). Oil contents of pumpkin ranged from 31.60% to 38.60%. The fatty acid compositions of seed oils were oleic (31.65%-43.43%), linoleic (37.62% - 49.25%), palmitic (9.98% - 12.41%) and stearic acids (6.20% - 7.28%). The content of linoleic acid was found higher than that of content of oleic acid of the genotypes except genotype 106. The antioxidant activities of seeds were found between 5.44% and 17.75%. Total phenol contents of seeds were determined between 0.25 and 0.35 mg GAE/ 100ml.