Every day, the evaluation and optimization become parts of our life, while the technologies have already maximized, because the raw resources become lowly and world needs increase on. A maximized growing medium for bakery yeast multiplication really means an insurance of the bread manufacturing’s future. Industrial medium supplementation with admixture stimulates the Saccharomyces cerevisiae growth when bakery yeast has to be produced. The stimulation is reflected by the generation time decrease and the biomass accumulation. The yeast produced on such medium present a higher fermentative capacity. The aim of this work was to optimization the growing medium used for the bakery yeast’s obtaining on laboratory level using factorial design and response surface methodology. Applying these methods allows the simultaneous analysis the effect of two (molasses and wheat embryos) and, respectively three factors (complex fertilizer, ammonium sulphate and magnesium sulphate) on the biomass accumulation.