The study investigated the chemical properties, fatty acid profile and vitamin content of Acacia seeds using the method of analysis of official analytical chemists (AOAC methods), gas chromatography and high performance liquid chromatography, respectively. The acid value and iodine value ranged from 23.3 to 53.2 g KOH / kg) and 1522.8 to 1878 g iodine / kg, respectively. Stearic and olic acid were the dominant fatty acids in the samples, whereas eicosenoic acid (C20:1) was highest in both A. elacantha and A. tumida.. Tocopherol ranged from 724 - 897 mg / kg, whereas α- and β- carotene and cryptoxanthin ranged from 965 to 1724, 974 to 1919, 650 to 2286 μg / 100g, respectively. Pyridoxine, thiamine, folate, niacin and ascorbic acid (μg / 100g)ranged from 109 - 301, 88 - 223, 41 - 91, 53 - 109 and 77 -250, respectively. The Acacia oils compared well with conventional oil and the seeds are nutritious.