Mycotoxin contamination of milk problem is the problem of current interest and has received great attention in the last three decades. It is known that the occurrence of aflatoxin M1 (AFM1) in milk is a public health problem, it has been recently classified by the International Agency for Research on Cancer as a possible human carcinogen factor. Because of this goal, the aim of this paper is to study some lactic cultures in order to reduce the content of AFM1 in milk contaminated. Results indicate significant differences (p <0.001) during the degradation AFM1 thermostat with mesophilic lactic cultures. Thus the potential of certain lactic cultures is promising and open new horizons of research in biotechnology decontamination AFM1.