The composition of volatile oil from Hüller Bitterer variety grown in Romania, was presented in our previous study. In this study chemometrics methods were applied for highlighting the statistical correlations existing between the genotype (variety), chemotype (composition), and phenotype (phenophase of cone development) in accordance with essential oils investigated. The essential oils content of hop cultivar was significantly dependent on the phenophases of the cones and types of samples (cones and pellets). Using the cluster analysis there have been highlighted major and minor volatile compounds present in hop variety. Principal component analysis provided the possibility of monitoring the accumulation of essential oil during the agricultural year, and at the same time, the differentiation of the phenophases of variety.