In the recent years the experts dealing with the healthy nutrition turned their attention towards the determination of the biological value of sprouts. Nowdays, the consumption of germinated seeds became popular also in Romania. This study aim was to determine the effect of light conditions on the content of sulfur-containing phytochemicals of different varieties of radish and mustard. For that, red radish, white radish, black radish, white mustard and black mustard seeds were germinated under 24 h light and 24 h dark conditions, at 23°C, for 7 days and glucosinolates along with S-methyl cysteine sulfoxide levels were determined and compared. These compounds are derived from amino acid biosynthesis and they appear to possess anticarcinogenic properties. Results showed that S-methyl cysteine sulfoxide is synthesised better under light condition meanwhile sprouts germinated in the dark, were characterized by a higher total glucosinolates content.