High thermal stability of sol-gel entrapped lipases

Cristina Paul, Paula Borza, Francisc Peter

Volume 21, Issue 2;
Pages: 173-180; 2015
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Lipases from Burkholderia cepacia (Amano PS) and Candida antarctica B (CalB) were immobilized by sol-gel entrapment techniques, using a ternary silane precursors system with vinyl and phenyl non-hydrolyzable groups. The influence of temperature on the catalytic properties of native and immobilized lipases was investigated by incubating the biocatalysts for 24 h at temperatures between 40ºC and 80ºC, in n-hexane medium. A long term stability study was made with immobilized CaLB biocatalysts by incubation for 120 h in isooctane at 80°C. The acylation of aliphatic sec-alcohols by vinyl acetate was used as model reaction. Thermogravimetric analysis (TGA) was employed to evaluate the thermal stability and degradation of the immobilized biocatalysts
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