Pesticides are poisons in nature and have harmful effects on human health. They are used for suicide, homicide and for other bad intentions. Objective: The aim of the study was to determine the residues of some pesticides in fruits collected from various markets of Lahore. Material & Methods: Eight fruits samples apple, banana, guava, melon, orange, papaya, pomegranate and strawberry from various sale points of the market were collected. Extraction, clean up and analysis were then processed. Concentration of five pesticides including Bifenthrin, Lambda Cyhalothrin, Carbofuran, Chlorpyrifos and Cypermethrin were determined in fruits samples using high performance thin layer chromatography. Spot diameter of pesticide residue of sample was compared with that of standards to determine pesticide concentration. One way ANOVA was applied to determine the significance of difference. Results & Cnoclusion: Significant difference of pesticide presence was identified in some selected fruits (P<0.05). The maximum concentration of cyhalothrin was found in Papaya (0.112 mg/kg) and Strawberry (0.133mg/kg), Cypermethrin in Orang (0.320 mg/kg), Banana (0.170 mg/kg) and Pomegranate (0.129 mg/kg), Chlorpyrifos in Guava (0.122 mg/kg), Carbofuran in Apple (0.109 mg/kg). It was concluded that cypermethrin possess high rate of contamination in different fruit samples. Therefore, there is a need to continuity of inspection on pesticide residues in agricultural products in order to prevent the contamination and secure human safety.