The internal validation of HPLC and conductometric method for the determination of hop bitter acids

Authors:
Liana-Claudia Salanţă, Maria Tofană, Sonia Socaci, Elena Mudura, Anca Fărcaş

Volume 19, Issue 3;
Pages: 362-366;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Abstract:
Conductometry and high-performance liquid chromatography (HPLC) methods are validated in the Food Safety and Quality Testing Laboratory (FQSL) of USAMV Cluj-Napoca. The determination of alpha and beta acids, from Magnum and Huller Bitterer variety, was carried out according to Analytica EBC, method 7.4 and 7.7. The aim of internal validation study was to assure quality results. For the HPLC method, the following quality parameters were determined: specificity, linearity, precision and accuracy. The obtained conductometric values for alpha bitter acids are similar with those obtained by HPLC analysis. Standard deviations were obtained in conditions of repeatability. The recovery coefficient determined for conductometric method was 92 % and for HPLC method was 95%.
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