Comparative study of potential cellulolytic and xylanolytic bacteria isolated from compost and their optimization for industrial use

Authors:
Nivedita Sharma, Preeti Buragohain, DivyaTandon, Richa Kaushal

Volume 19, Issue 3;
Pages: 284-297;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Abstract:
Compost a nutrient rich organic fertilizer and soil conditioner is a product of humification of organic matter. Compost formation is aided by combination of living organisms including bacteria, fungi and worms which transform lignocellulosic waste into humic like substances. Interest in microorganisms responsible for degrading compost has increased due to potential commercial applications which include biodegradation and production of their bioactive compounds such as enzymes. So the present study was carried out to isolate celluloytic and xylanolytic microorganisms from rural compost and their screening for potential hydrolytic microorganisms. In total, 49 isolates were isolated from compost, out of which 43 were bacteria. Among all the isolates, four bacterial isolates were selected for enzyme production studies depending upon their higher cellulase (CMCase, FPase and β-glucosidase) and xylanase producing capability. Out of selected isolates two were K22, K23, R5& N11 were identified as B. licheniformis, B. sps.B.megateriumandB. subtilisN11 respectively by physiological, biochemical and 16 S rRNA PCR technique.
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