Production, purification and characterization of cellulase free-xylanase by Bacillus coagulans B30 using lignocellulosic forest wastes with different pretreatment methods

Authors:
Nivedita Sharma, Shelly Ranchan, Shruti Pathania

Volume 19, Issue 1;
Pages: 28-36;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551

Bacillus coagulans B30 isolated from forest soil of northern Himalayan of India, explored for its cellulase free-xylanase production using physio-chemically pretreated forest biomass as a substrate. Maximum xylanase production was observed in microwave pretreated mixed biomass producing 281.63U/g of xylanase under solid state fermentation. Xylanase was purified having molecular weight of 66.54 kDa by using SDS Page. Purified xylanase showed high temperature (50C) and pH (9.00) optimum.