Evaluation of different forest wastes of Northern Himalayas as a potential substrate for cellulase production by Aspergillus niger F7 and its partial purification

Authors:
Nivedita Sharma, Shavi Mahajan, Nisha Sharma

Volume 18, Issue 4;
Pages: 324-335;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551
Abstract:
Biodegradation of lignocellulosic forest waste by Aspergillus niger F7 under solid state fermentation was explored. Different pretreatments were given to render forest wastes readily accessible to the enzymatic attack. SSF of pretreated forest biomass was found to be superior over untreated forest biomass. Pretreated forest biomass has emerged as a suitable substrate for cellulase production by A. niger F7 reaching upto 146.14 U/g in acid+ steam pretreated P. roxburghii needles. It has also been noticed that though forest lignocellulosics when used as carbon source yielded fairly good amount of cellulase. The ultimate goal of the study is the efficient utilization of wastes so as to recover cellulase in concentrations that make purification feasible. The data gathered in this study provides us a glimpse of some of the dynamics of the production of cellulase.
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