Biochemical studies on the protective effects of Egyptian Montmorillonite clay and Activated Carbon against health hazards resulting from the exposure to Deoxynivalenol mycotoxin in food

Authors:
Mohamed M. Sief, Mona M. Abdel-Galil, Farouk A. Khalil, Abdel-Hady F. Hussein

Volume 18, Issue 4;
Pages: 283-295;
ISSN: 2069-0053 (print) (former ISSN: 1453-1399), Agroprint;
ISSN (online): 2068-9551

The current study aimed to use the Egyptian Montmorillonite clay (EM clay) and Activated Carbon (AC) derived from dates stones as a protective agent against Deoxynivalenol toxicity. Forty eight female Sprague-Dawley rats were divided into six groups including the control group, the group fed basal diet plus EM clay (0.5%), the group fed basal diet plus AC (0.5) % and other three groups fed DON- contaminant diet (25 mg / kg diet) alone or plus EM clay (0.5 %) or plus AC (0.5%). At the end of the experimental period, blood samples were collected for serum biochemical analyses. Animals were scarified and dissected samples of liver and kidney were collect for histopathology and determination of antioxidant enzyme activities and lipid peroxidation in liver. The results indicated that rats fed DON-contaminated diet showed a significant increase in the activities of ALT, AST, ATP and levels of uric acid, createnin, total lipids, triglycerides and lipid peroxidation and also showed significant decrease in Total Antioxidant Capacity (TAC), Nitric oxide activities and significant decreases in GSH level. Histological examination of the liver and kidney tissues showed severe histopathological and histochemical changes. Animals fed DONcontaminated diet plus EM clay or AC showed a significant elimination of the harmful effects of DON in all biochemical, antioxidant parameters and the histological picture of liver and kidney.