Aflatoxins are carcinogenic substances produced by fungi and due to the growth of fungi in a wide range of commodities including cereals, nuts, figs and spices, it is necessary to test such products for the presence of aflatoxins prior to their incorporation into other foodstuff or feeds. There is a need for a quick, simple test which can be used to check a diverse range of foodstuff. Two different detection methods of aflatoxins in peanuts were presented and compared (HPLC and ELISA). Both methods allow the determination of aflatoxin content with good recoveries for the levels of interest.