The aim of the research is to study the antioxidant activity and concentration of phenolic substances with
antioxidant role in milling fractions of two characteristic cultivars of Romanian oat. The method Folin –
Ciocâlteu has been used to determine the total content of phenolic substances. The total antioxidant activity
was determined by spectrophotometric method using the reagent DPPH (2,2-Diphenyl-1-Picryl Hydrasil),
as system of releasing the free radical DPPH. The antioxidant activity is expressed as antiradical activity of
samples and as percentage. Both cultivars analyzed have registered the highest content of phenolic
substances in bran, and the antioxidant capacity of the fractions analyzed has been strongly correlated with
the content of phenolic substances and that of ash. The data resulted from the experiment confirm the
positioning of substances with antioxidant role especially in the superior strata of oat grain and high
quantity of substances with antioxidant role in oat.