A trout diet was supplemented with 0, 8.5, or 15 g/100 g of flaxseed oil (FO). To prevent lipid oxidation of
fillets, FO-supplemented diets were also enhanced with 0, 400, and 900 mg/kg of alpha-tocopheryl acetate
(α-TA). α-tocopherol content of fillets were determined following fish harvest on days 0, 30, 60, 90, and
120. FO supplementation resulted in increased (P<0.05) concentration of omega-3 fatty acid (ω3 FA) in
fillets, mainly due almost two-fold increase (P<0.05) of α-linolenic acid, while docosahexaenoic and
eicopentaenoic acids slightly decreased (P<0.05). The highest (P<0.05) α-tocopherol content in fillets was
determined when supplementing trout with 900 mg/kg of α-TA at day 120. Our results indicate that
regardless of FO level in trout diet, 900 mg/kg of α-TA can prevent lipid deterioration of fillets.