Superoxide Dismutase Activity in the freshwater crustacean Daphnia magna exposed to Pb(II)

P. Peppos, A. Salifoglou, S. Hadjispyrou

Volume 17, Issue 2;
Pages: 116-118;
ISSN: 2069-0053 (print) (former: ISSN:1453-1399), Agroprint;
ISSN (online):2068 – 9551
The aim of this work was the study of the toxic action of 2-hydroxyethyl-iminodiacetic Pb(II), [Pb – (HEIDA)] on the freshwater index-organism Daphnia magna. The results obtained from the toxicity experiments have been processed through the Trevors program, in the environment of BASIC, in order to assess the toxicity of the Pb(II) complex, expressed as EC50-48h. Based on the final results, it was found, that the Pb(II) complex had an EC50-48h value of 4.46 mg/L, leading to the conclusion that it is toxic enough to Daphnia magna.

In order to confirm that all experimental procedures were carried out properly, a reference experiment was conducted, using potassium dichromate as a toxic substance. The toxicity of potassium dichromate, was found to be within the acceptable limits, issued by ISO 6341, i.e. 0.85 mg/L (ppm).

Following determination of the EC50-48h of the Pb(II) complex, the study of its influence on the activity of the antioxidant enzyme Superoxide Dismutase of Copper - Zinc (Cu,Zn- SOD), isolated properly from the organism, was conducted. From the results of this study, it was found that the Pb(II) complex has practically no influence on the enzyme activity.
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