The aim of this study was to evaluate the metallic contamination of Melampyrum sp. extract samples. Chemical characterization of Melampyrum sp. extract was performed by measuring metal content in samples from three different sites. The sites were chosen based on informations general, for were contaminated by heavy metals. Melampyrum sp. Extracts, prepared from spectrophotometric determination, with standard metal solutions obtained in accordance with ISO 11466 with atomic absorption spectrometry method. Melampyrum sp. extracts, samples presented high contents of copper (Cu), cadmium (Cd), manganese (Mn), Iron (Fe), Hg, Mo, Pb, Zn, Cr and nickel (Ni). Amounts of heavy metals above the admitted limit by the OMS have been discovered. The content of heavy metals in Melampyrum sp. extract was generally within safe limits, and compared well with levels in foods from Food Codex, though some metals contents were high.