Biofilms can be defined as communities of microorganisms attached to a surface. The purpose of this work was to investigate the roles of strain diversity and environmental conditions in biofilm formation by Bacillus subtilis, Bacillus cereus and Pseudomonas fluorescens using various assays and culture conditions and a range of strains.
Experiments were achieved using several protocols based on comparative analysis studies. The physical and biological parameters effects, strain of bacteria, substrate composition and temperature on biofilm formation and evolution were analyzed. The results in this article indicate that tested bacterial strains can form monospecies or mixed biofilms and can behave physiologically different in biofilm formation.