The paper proposes the anthocyanins extraction from Vaccinium Myrtillus L. frozen fruits. Experiments were performed in solvents like methanol, ethanol using two different extraction methods. The extracts have been analyzed by UV-VIS technique, FT-IR and high-performance liquid chromatography (HPLC) using a Dionex Ultimate 3000 apparatus equipped with photodiode array detector for characterized the anthocyanins. It has been identified a number of fifteen anthocyanins in bilberries extracts. Qualitative composition of anthocyanidins was performed after acidic hydrolysis of extracts.