In this study was followed how storage temperature and storage time affect fatty acids profile of infant formula. Storage conditions chosen were: room temperature and 15°C. Samples of infant formula were deposited during 18 months, which correspond to infant formula shelf life; the samples were analyzed at obtaining and after 3, 6, 9, 12, 15, and 18 months of storage at conditions mentioned before. For statistical analysis were used One-way ANOVA with Tukey Multiple Comparison post Test (for the effect of time on fatty acids profile during storage) and with Paired t Test (for the effect of temperature).