Antioxidant activity of some celandine (Chelidonium majus L.) carotenoidic extracts Delia G.

Authors:
Delia G. Dumbravă, Nicoleta G. Hadarugă, Daniel I. Hadarugă, Alexandra Gruia, Calin Tatu, Virgil Păunescu, Alfa X. Lupea
Abstract:
The paper presents the antioxidant activity determination of some celandine (Chelidonium majus L.) carotenoidic extracts, using a method with 2,2-diphenyl-1-picrylhidrazyl (DPPH) free radical and the results correlation with the carotenoids content. The carotenoidic extracts obtained from celandine leaves, flowers and stem was analysed by reverse phase – high performance liquid chromatography (RP-HPLC), in view of β-carotene and total carotenoids content determination, using an Agilent 1100 RP-HPLC system with a Zorbax SB-C18 column. The highest carotenoids content was found in the celandine flowers 1377.70 µg/g, followed by the stem (1041.11 µg/g) and leaves (1007.06 µg/g). Celandine flowers also contain the greatest β-carotene amounts (369.50 µg/g), the smallest content being in stem (53.30 µg/g). The obtained data concerning the carotenoidic extracts antioxidant activity was compared with those of the ascorbic acid at different concentration (0.1 mM, 0.01 mM and 0.001 mM) , as antioxidant standard. The best antioxidant activity presents the celandine flowers extract and the smallest, the stem extract, the results being propotionally with the extracts carotenoids concentration.
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