Antioxidant activity of some celandine (Chelidonium majus L.) carotenoidic extracts Delia G.

Delia G. Dumbravă, Nicoleta G. Hadarugă, Daniel I. Hadarugă, Alexandra Gruia, Calin Tatu, Virgil Păunescu, Alfa X. Lupea
The paper presents the antioxidant activity determination of some celandine (Chelidonium majus L.) carotenoidic extracts, using a method with 2,2-diphenyl-1-picrylhidrazyl (DPPH) free radical and the results correlation with the carotenoids content. The carotenoidic extracts obtained from celandine leaves, flowers and stem was analysed by reverse phase – high performance liquid chromatography (RP-HPLC), in view of β-carotene and total carotenoids content determination, using an Agilent 1100 RP-HPLC system with a Zorbax SB-C18 column. The highest carotenoids content was found in the celandine flowers 1377.70 µg/g, followed by the stem (1041.11 µg/g) and leaves (1007.06 µg/g). Celandine flowers also contain the greatest β-carotene amounts (369.50 µg/g), the smallest content being in stem (53.30 µg/g). The obtained data concerning the carotenoidic extracts antioxidant activity was compared with those of the ascorbic acid at different concentration (0.1 mM, 0.01 mM and 0.001 mM) , as antioxidant standard. The best antioxidant activity presents the celandine flowers extract and the smallest, the stem extract, the results being propotionally with the extracts carotenoids concentration.
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