Microorganisms are forming structurally complex multicellular communities (biofilms) by production of an extracellular matrix. The development of biofilm in food processing area determines microbial contamination of processed food. This biofilm may contain spoilage microorganisms or/and pathogenic microorganisms. This work presumes the study of microbial adhesion on different material used in food processing. The experiments are developing within a 20 day period. Adhesion was analyzed at variable temperature (8ºC, 20ºC, 37ºC), the result being formation of a mature biofilm. We used different coupons of food-use approved materials (wood, glass, stainless steel, and polytetrafluoroethylene). We analyzed microorganism adhesion, as well as time and temperature influence on biofilm formation.