Milk and milk products represent the subject for numerous tests regarding public safety assurance and in the same time following standard composition. More, together with our country official entrance in European Community, the major objective is food security improvement and animal health developing in ecologic breeding systems. This paper present the introduction of a new modern analysis method, for screening the possible presence of antibiotics residues in milk and milk products, and also the evaluation of method selectivity and sensitivity, in accordance with ISO EN 17025/2005 stipulations.