The presence of titanium in chemical and biological systems has been amply emphasized over the past decades. The use of that early transition metal in widespread medical and agricultural applications necessitates the perusal of its related chemistry with variable size biological substrates. Keeping in mind that interactions of titanium with cellular components are key to understanding its role in a complex biological world, an in-depth investigation was carried out on the speciation chemistry of the binary Ti(IV)-citric acid system in aqueous media.