The biology of yeasts cell is in a tight connection with the existence conditions. The most important environment factors its report on chemical composition of medium, especially the concentration of different nutrients (Nam Sum Wang, 2000). For yeasts the most important microingredients are vitamins. Tacking this in consideration we can tell that vitamins could be decisive for yeasts growth and for a real increasing of enzymes activity, too. The scope of experiments was to establish the influence of different quantity of vitamins upon the invertase activity of yeasts.